Phospholipase C from genetically modified Pichia pastoris (CTA) 2008 - Page 1(7) PHOSPHOLIPASE C EXPRESSED IN PICHIA PASTORIS
نویسنده
چکیده
Phospholipase C catalyzes the hydrolysis of the sn-3 phosphate head group in phospholipids including phosphatidylcholine, phosphatidyletanolamine, and phosphatidylserine with the formation of 1,2-diacylglycerol and water soluble phosphate esters phosphorylcholine, phosphorylethanolamine, and phosphorylserine. Phospholipase C from P. pastoris is intended for use in the refining of soybean oil and other vegetable oils used in food (Ciofalo et al., 2006). The enzyme would be added to the crude oil at levels sufficient to hydrolyse phospholipids. The resulting phosphate esters would be removed from the oil, while 1,2-diacylglycerol would remain in the oil. The residues of phospholipase C would also be removed from the oil during subsequent purification steps.
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